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Use of real-time quantitative PCR to detect Chlamydophila felis infection.


ABSTRACT: A real-time PCR assay was developed to detect and quantify Chlamydophila felis infection of cats. The assay uses a molecular beacon to specifically identify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies.

SUBMITTER: Helps C 

PROVIDER: S-EPMC88209 | biostudies-literature | 2001 Jul

REPOSITORIES: biostudies-literature

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Use of real-time quantitative PCR to detect Chlamydophila felis infection.

Helps C C   Reeves N N   Tasker S S   Harbour D D  

Journal of clinical microbiology 20010701 7


A real-time PCR assay was developed to detect and quantify Chlamydophila felis infection of cats. The assay uses a molecular beacon to specifically identify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies. ...[more]

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