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Investigation of discordant SARS-CoV-2 RT-PCR results using minimally processed saliva.

ABSTRACT: Saliva is an attractive sample for coronavirus disease 2019 testing due its ease of collection and amenability to detect viral RNA with minimal processing. Using a direct-to-RT-PCR method with saliva self-collected from confirmed COVID-19 positive volunteers, we observed 32% false negative results. Confirmed negative and healthy volunteer samples spiked with 10⁶ genome copies/mL of heat-inactivated severe acute respiratory syndrome coronavirus 2 showed false negative results of 10% and 13%, respectively. Additional sample heating or dilution of the false negative samples conferred only modest improvements. These results highlight the potential to significantly underdiagnose COVID-19 infections when testing directly from minimally processed heterogeneous saliva samples.

SUBMITTER: White D

PROVIDER: S-EPMC8857222 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Investigation of discordant SARS-CoV-2 RT-PCR results using minimally processed saliva.

White Dawn D Gu Jimmy J Steinberg Catherine-Jean CJ Yamamura Deborah D Salena Bruno J BJ Balion Cynthia C Filipe Carlos D M CDM Capretta Alfredo A Li Yingfu Y Brennan John D JD

Scientific reports 20220218 1

Saliva is an attractive sample for coronavirus disease 2019 testing due its ease of collection and amenability to detect viral RNA with minimal processing. Using a direct-to-RT-PCR method with saliva self-collected from confirmed COVID-19 positive volunteers, we observed 32% false negative results. Confirmed negative and healthy volunteer samples spiked with 10<sup>6</sup> genome copies/mL of heat-inactivated severe acute respiratory syndrome coronavirus 2 showed false negative results of 10% an ...[more]

PMID: 35181678

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2021-04-22 | GSE173086 | GEO

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