Dataset Information

The induced membrane technique in animal models: a systematic review.

ABSTRACT:

Objectives

The induced membrane technique (IMT) is a 2-stage surgical approach that has become increasingly popular to manage bone defects. Preclinical investigations have been conducted to better understand and define several aspects of this technique. This review summarizes the literature regarding the IMT performed in animal models and identifies potential future directions.

Data sources

Biosis Citation Index, Ovid Embase, and Ovid MEDLINE databases were searched from inception up to June 23, 2021 for articles related to the IMT.

Study selection

Animal studies involving the use of the IMT for segmental defects in long bones were selected. Only full-length original research articles published in English or French were included.

Data extraction

Two authors extracted the data from the selected studies and a third author verified the accuracy of the information.

Data synthesis

Information concerning the animal model, the surgical procedures, and the outcome measures were recorded for each study and compiled.

Conclusions

Forty-seven studies were included in this review. Twenty-nine studies (62%) performed both stages of the technique, but only 8 (17%) reported on radiographic union rates explicitly and 5 (11%) included biomechanical testing. A large proportion of the preclinical literature on the IMT has failed to report on radiographic union as an outcome. While studies reporting membrane properties are valuable, they may not provide information that translates into clinical practice or further clinical research if the ultimate outcome of bony healing is not considered. Future animal studies of the IMT should consider this in their study design.

SUBMITTER: Sun H

PROVIDER: S-EPMC8900461 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The induced membrane technique in animal models: a systematic review.

Sun Hening H Godbout Charles C Hali Kalter K Momic Jovana J Schemitsch Emil H EH Nauth Aaron A

OTA international : the open access journal of orthopaedic trauma 20220310 1 Suppl

<h4>Objectives</h4>The induced membrane technique (IMT) is a 2-stage surgical approach that has become increasingly popular to manage bone defects. Preclinical investigations have been conducted to better understand and define several aspects of this technique. This review summarizes the literature regarding the IMT performed in animal models and identifies potential future directions.<h4>Data sources</h4>Biosis Citation Index, Ovid Embase, and Ovid MEDLINE databases were searched from inception ...[more]

PMID: 35282388

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Project description:BackgroundAnimal models are widely used to study pathological processes and drug (side) effects in a controlled environment. There is a wide variety of methods available for establishing animal models depending on the research question. Commonly used methods in tumor research include xenografting cells (established/commercially available or primary patient-derived) or whole tumor pieces either orthotopically or heterotopically and the more recent genetically engineered models-each type with their own advantages and disadvantages. The current systematic review aimed to investigate the meningioma model types used, perform a meta-analysis on tumor take rate (TTR), and perform critical appraisal of the included studies. The study also aimed to assess reproducibility, reliability, means of validation and verification of models, alongside pros and cons and uses of the model types.MethodsWe searched Medline, Embase, and Web of Science for all in vivo meningioma models. The primary outcome was tumor take rate. Meta-analysis was performed on tumor take rate followed by subgroup analyses on the number of cells and duration of incubation. The validity of the tumor models was assessed qualitatively. We performed critical appraisal of the methodological quality and quality of reporting for all included studies.ResultsWe included 114 unique records (78 using established cell line models (ECLM), 21 using primary patient-derived tumor models (PTM), 10 using genetically engineered models (GEM), and 11 using uncategorized models). TTRs for ECLM were 94% (95% CI 92-96) for orthotopic and 95% (93-96) for heterotopic. PTM showed lower TTRs [orthotopic 53% (33-72) and heterotopic 82% (73-89)] and finally GEM revealed a TTR of 34% (26-43).ConclusionThis systematic review shows high consistent TTRs in established cell line models and varying TTRs in primary patient-derived models and genetically engineered models. However, we identified several issues regarding the quality of reporting and the methodological approach that reduce the validity, transparency, and reproducibility of studies and suggest a high risk of publication bias. Finally, each tumor model type has specific roles in research based on their advantages (and disadvantages).Systematic review registrationPROSPERO-ID CRD42022308833.

Dataset Information

The induced membrane technique in animal models: a systematic review.

Objectives

Data sources

Study selection

Data extraction

Data synthesis

Conclusions

Publications

The induced membrane technique in animal models: a systematic review.

Similar Datasets

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