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Dependence of click-SELEX performance on the nature and average number of modified nucleotides.

ABSTRACT: The click-SELEX procedure enables the identification of nucleobase-modified aptamers in which chemical entities are introduced by a copper(i)-catalysed alkyne-azide 'click' reaction. Here we report on the impact of modified nucleobases on PCR conditions and the average amount of modified nucleobases on click-SELEX performance. We demonstrate click-SELEX being strongly dependent on which and on how many modifications are used. However, when using C3-GFP the number of modifications did not impact the overall success of the selection procedure.

SUBMITTER: Siegl J

PROVIDER: S-EPMC8905535 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Dependence of click-SELEX performance on the nature and average number of modified nucleotides.

Siegl Julia J Plückthun Olga O Mayer Günter G

RSC chemical biology 20220221 3

The click-SELEX procedure enables the identification of nucleobase-modified aptamers in which chemical entities are introduced by a copper(i)-catalysed alkyne-azide 'click' reaction. Here we report on the impact of modified nucleobases on PCR conditions and the average amount of modified nucleobases on click-SELEX performance. We demonstrate click-SELEX being strongly dependent on which and on how many modifications are used. However, when using C3-GFP the number of modifications did not impact ...[more]

PMID: 35359492

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