Unknown

Dataset Information

0

Pulmonary fibrosis model using micro-CT analyzable human PSC-derived alveolar organoids containing alveolar macrophage-like cells.


ABSTRACT: Human lung organoids (hLOs) are useful for disease modelling and drug screening. However, a lack of immune cells in hLOs limits the recapitulation of in vivo cellular physiology. Here, we generated hLOs containing alveolar macrophage (AMφ)-like cells derived from pluripotent stem cells (PSC). To bridge hLOs with advanced human lung high-resolution X-ray computed tomography (CT), we acquired quantitative micro-CT images. Three hLO types were observed during differentiation. Among them, alveolar hLOs highly expressed not only lung epithelial cell markers but also AMφ-specific markers. Furthermore, CD68+ AMφ-like cells were spatially organized on the luminal epithelial surface of alveolar hLOs. Bleomycin-treated alveolar hLOs showed upregulated expression of fibrosis-related markers and extracellular matrix deposits in the alveolar sacs. Alveolar hLOs also showed structural alterations such as excessive tissue fraction under bleomycin treatment. Therefore, we suggest that micro-CT analyzable PSC-derived alveolar hLOs are a promising in vitro model to predict lung toxicity manifestations, including fibrosis.

SUBMITTER: Choi S 

PROVIDER: S-EPMC8907399 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8684607 | biostudies-literature
| S-EPMC8693665 | biostudies-literature
| S-EPMC7141408 | biostudies-literature
| S-EPMC7961057 | biostudies-literature
| S-EPMC1899274 | biostudies-other
2021-11-19 | GSE172123 | GEO
| S-EPMC8417529 | biostudies-literature
2014-06-30 | GSE49072 | GEO
2021-11-19 | GSE172121 | GEO
2021-11-19 | GSE172122 | GEO