Ontology highlight
ABSTRACT:
SUBMITTER: Sadygov RG
PROVIDER: S-EPMC8921656 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Briefings in bioinformatics 20220301 2
Protein turnover is vital for cellular functioning and is often associated with the pathophysiology of a variety of diseases. Metabolic labeling with heavy water followed by liquid chromatography coupled to mass spectrometry is a powerful tool to study in vivo protein turnover in high throughput and large scale. Heavy water is a cost-effective and easy to use labeling agent. It labels all nonessential amino acids. Due to its toxicity in high concentrations (20% or higher), small enrichments (8% ...[more]