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An SHV-derived extended-spectrum beta-lactamase in Pseudomonas aeruginosa.

ABSTRACT: A clinical isolate of Pseudomonas aeruginosa RP-1 produced the extended-spectrum beta-lactamase (ESBL) SHV-2a. Its gene was expressed from a composite promoter made of the -35 region derived from the left inverted repeat of IS26 and the -10 region from the blaSHV-2a promoter itself. The DNA sequences immediately surrounding blaSHV-2a were homologous to plasmid pMPA2a from Klebsiella pneumoniae KpZU-3, while further away and 3' to the blaSHV-2a gene, a sequence corresponding to the left end of Tn1721 was detected, thus indicating a likely enterobacterial origin of this ESBL gene.

SUBMITTER: Naas T

PROVIDER: S-EPMC89260 | biostudies-literature | 1999 May

REPOSITORIES: biostudies-literature

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An SHV-derived extended-spectrum beta-lactamase in Pseudomonas aeruginosa.

Naas T T Philippon L L Poirel L L Ronco E E Nordmann P P

Antimicrobial agents and chemotherapy 19990501 5

A clinical isolate of Pseudomonas aeruginosa RP-1 produced the extended-spectrum beta-lactamase (ESBL) SHV-2a. Its gene was expressed from a composite promoter made of the -35 region derived from the left inverted repeat of IS26 and the -10 region from the blaSHV-2a promoter itself. The DNA sequences immediately surrounding blaSHV-2a were homologous to plasmid pMPA2a from Klebsiella pneumoniae KpZU-3, while further away and 3' to the blaSHV-2a gene, a sequence corresponding to the left end of Tn ...[more]

PMID: 10223953

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