Ontology highlight
ABSTRACT:
SUBMITTER: Onodera Y
PROVIDER: S-EPMC89495 | biostudies-literature | 1999 Oct
REPOSITORIES: biostudies-literature
Antimicrobial agents and chemotherapy 19991001 10
The genes encoding the DNA gyrase A and B subunits of Bacteroides fragilis were cloned and sequenced. The gyrA and gyrB genes code for proteins of 845 and 653 amino acids, respectively. These proteins were expressed in Escherichia coli, and the combination of GyrA and GyrB exhibited ATP-dependent supercoiling activity. To analyze the role of DNA gyrase in quinolone resistance of B. fragilis, we isolated mutant strains by stepwise selection for resistance to increasing concentrations of levofloxa ...[more]