Ontology highlight
ABSTRACT:
SUBMITTER: Morjen M
PROVIDER: S-EPMC8955015 | biostudies-literature | 2022 Feb
REPOSITORIES: biostudies-literature
Morjen Maram M Moslah Wassim W Touihri-Baraketi Imen I Srairi-Abid Najet N Luis José J Marrakchi Naziha N Jebali Jed J
Toxins 20220225 3
PIVL is a Kunitz-type serine protease inhibitor that was previously characterized from Tunisian snake venom, <i>Macrovipera lebetina transmediterranea</i>. It reduced glioblastoma cells' development and significantly blocked angiogenesis in in-vitro and ex-vivo models. PIVL exerted these effects by interfering with αvβ3 integrin. In order to produce a biological active recombinant, the cDNA cloning and expression of PIVL was performed in <i>Escherichia coli</i> (BL21)-DE3 cells using pET-22b (+) ...[more]