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Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping.

ABSTRACT: We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 198 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 283 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.

SUBMITTER: Liu Y

PROVIDER: S-EPMC8978952 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping.

Liu Yang Y DiStasio Marcello M Su Graham G Asashima Hiromitsu H Enninful Archibald A Qin Xiaoyu X Deng Yanxiang Y Bordignon Pino P Cassano Marco M Tomayko Mary M Xu Mina M Halene Stephanie S Craft Joseph E JE Hafler David D Fan Rong R

Research square 20220330

We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 198 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 283 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research. ...[more]

PMID: 35378748

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