Dataset Information
Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing.
Ontology highlight
ABSTRACT: Objectives
Until recently, communication between neighboring cells in islets of Langerhans was overlooked by genomic technologies, which require rigorous tissue dissociation into single cells.Methods
We utilize sorting of physically interacting cells (PICs) with single-cell RNA-sequencing to systematically map cellular interactions in the endocrine pancreas after pancreatectomy.Results
The pancreas cellular landscape features pancreatectomy associated heterogeneity of beta-cells, including an interaction-specific program between paired beta and delta-cells.Conclusions
Our analysis suggests that the particular cluster of beta-cells that pairs with delta-cells benefits from stress protection, implying that the interaction between beta- and delta-cells might safeguard against pancreatectomy associated challenges. The work encourages testing the potential relevance of physically-interacting beta-delta-cells also in diabetes mellitus.
SUBMITTER: Yanowski E
PROVIDER: S-EPMC8983436 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing.
Molecular metabolism 20220301
<h4>Objectives</h4>Until recently, communication between neighboring cells in islets of Langerhans was overlooked by genomic technologies, which require rigorous tissue dissociation into single cells.<h4>Methods</h4>We utilize sorting of physically interacting cells (PICs) with single-cell RNA-sequencing to systematically map cellular interactions in the endocrine pancreas after pancreatectomy.<h4>Results</h4>The pancreas cellular landscape features pancreatectomy associated heterogeneity of bet ...[more]
