Dataset Information

Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation.

ABSTRACT: The cerebral cortex has a number of conserved morphological and functional characteristics across brain regions and species. Among them, the laminar differences in microvascular density and mitochondrial cytochrome c oxidase staining suggest potential laminar variability in the baseline O₂ metabolism and/or laminar variability in both O₂ demand and hemodynamic response. Here, we investigate the laminar profile of stimulus-induced intravascular partial pressure of O₂ (pO2) transients to stimulus-induced neuronal activation in fully awake mice using two-photon phosphorescence lifetime microscopy. Our results demonstrate that stimulus-induced changes in intravascular pO₂ are conserved across cortical layers I-IV, suggesting a tightly controlled neurovascular response to provide adequate O₂ supply across cortical depth. In addition, we observed a larger change in venular O₂ saturation (ΔsO₂) compared to arterioles, a gradual increase in venular ΔsO₂ response towards the cortical surface, and absence of the intravascular "initial dip" previously reported under anesthesia. This study paves the way for quantification of layer-specific cerebral O₂ metabolic responses, facilitating investigation of brain energetics in health and disease and informed interpretation of laminar blood oxygen level dependent functional magnetic resonance imaging signals.

SUBMITTER: Sencan I

PROVIDER: S-EPMC8985437 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation.

Şencan İkbal İ Esipova Tatiana T Kılıç Kıvılcım K Li Baoqiang B Desjardins Michèle M Yaseen Mohammad A MA Wang Hui H Porter Jason E JE Kura Sreekanth S Fu Buyin B Secomb Timothy W TW Boas David A DA Vinogradov Sergei A SA Devor Anna A Sakadžić Sava S

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 20200609 3

The cerebral cortex has a number of conserved morphological and functional characteristics across brain regions and species. Among them, the laminar differences in microvascular density and mitochondrial cytochrome c oxidase staining suggest potential laminar variability in the baseline O2 metabolism and/or laminar variability in both O2 demand and hemodynamic response. Here, we investigate the laminar profile of stimulus-induced intravascular partial pressure of O2</s ...[more]

PMID: 32515672

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Project description:New findingsWhat is the central question of this study? Contrast-enhanced ultrasound (CEUS) can be used to directly assess skeletal muscle perfusion but its day-to-day repeatability over time has not yet been validated: is CEUS a repeatable method for the measurement of skeletal muscle microvascular blood flow (MBF) at rest and in response to exercise, across independent assessment sessions? What is the main finding and its importance? A strong agreement between CEUS MBF measures across sessions suggests it is a repeatable method for assessing skeletal muscle perfusion over time. This validation provides confidence for incorporating these measures into longitudinal studies such as a chronic intervention or disease progression to gain further knowledge of skeletal muscle microvascular function.AbstractContrast-enhanced ultrasound (CEUS) can be used to directly assess skeletal muscle perfusion. However, its repeatability over time has not yet been validated and therefore its use in longitudinal measures (i.e., exploring the impact of a chronic intervention or disease progression) is limited. This study aimed to determine the repeatability of CEUS for the measurement of skeletal muscle microvascular blood flow (MBF) at baseline and in response to exercise, across independent assessment sessions. Ten healthy volunteers (five female; 30 ± 6 years) had CEUS of the right vastus lateralis recorded in two separate sessions, 14 days apart. Measurements were taken at baseline, during an isometric leg extension and during recovery. Acoustic intensity data from a region of interest were plotted as a replenishment curve to obtain blood volume (A) and flow velocity (β) values from a one-phase association non-linear regression of mean tissue echogenicity. Linear regression and Bland-Altman analyses of A and β values were performed, with significance assumed as P < 0.05. Strong positive correlations were observed across sessions for all A and β values (both P < 0.0001). Bland-Altman analysis showed a bias (SD) of -0.013 ± 1.24 for A and -0.014 ± 0.31 for β. A bias of 0.201 ± 0.770 at baseline, 0.527 ± 1.29 during contraction and -0.203 ± 1.29 at recovery was observed for A, and -0.0328 ± 0.0853 (baseline), -0.0446 ± 0.206 (contraction) and 0.0382 ± 0.233 (recovery) for β. A strong agreement between CEUS MBF measures across independent sessions suggests it to be a repeatable method for assessing skeletal muscle perfusion over time, and therefore facilitates wider use in longitudinal studies.

Dataset Information

Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation.

Publications

Optical measurement of microvascular oxygenation and blood flow responses in awake mouse cortex during functional activation.

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