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Retroviral RNase H: Structure, mechanism, and inhibition.

ABSTRACT: All retroviruses encode the enzyme, reverse transcriptase (RT), which is involved in the conversion of the single-stranded viral RNA genome into double-stranded DNA. RT is a multifunctional enzyme and exhibits DNA polymerase and ribonuclease H (RNH) activities, both of which are essential to the reverse-transcription process. Despite the successful development of polymerase-targeting antiviral drugs over the last three decades, no bona fide inhibitor against the RNH activity of HIV-1 RT has progressed to clinical evaluation. In this review article, we describe the retroviral RNH function and inhibition, with primary consideration of the structural aspects of inhibition.

SUBMITTER: Ilina TV

PROVIDER: S-EPMC8994160 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Retroviral RNase H: Structure, mechanism, and inhibition.

Ilina Tatiana V TV Brosenitsch Teresa T Sluis-Cremer Nicolas N Ishima Rieko R

The Enzymes 20210924

All retroviruses encode the enzyme, reverse transcriptase (RT), which is involved in the conversion of the single-stranded viral RNA genome into double-stranded DNA. RT is a multifunctional enzyme and exhibits DNA polymerase and ribonuclease H (RNH) activities, both of which are essential to the reverse-transcription process. Despite the successful development of polymerase-targeting antiviral drugs over the last three decades, no bona fide inhibitor against the RNH activity of HIV-1 RT has prog ...[more]

PMID: 34861939

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