Dataset Information

The mid-secretory endometrial transcriptomic landscape in endometriosis: a meta-analysis.

ABSTRACT:

SUBMITTER: Vargas E

PROVIDER: S-EPMC9022214 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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The mid-secretory endometrial transcriptomic landscape in endometriosis: a meta-analysis.

Vargas E E García-Moreno E E Aghajanova L L Salumets A A Horcajadas J A JA Esteban F J FJ Altmäe S S

Human reproduction open 20220404 2

<h4>Study question</h4>Do women with endometriosis have a different endometrial gene expression profile at the time of embryo implantation than women without endometriosis?<h4>Summary answer</h4>The endometrial gene expression profile of women with endometriosis differs from that of women without endometriosis at the mid-secretory phase, although the differences are small.<h4>What is known already</h4>About 50% of women with endometriosis suffer infertility. Several molecular studies have sugges ...[more]

PMID: 35464885

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Project description:BackgroundCompromised receptivity of the endometrium is a major cause of unexplained infertility, implantation failure and subclinical pregnancy loss. In order to investigate the changes in endometrial protein profile as a cause of unexplained infertility, the current study was undertaken to analyze the differentially expressed proteins of endometrium from early-secretory (LH+2) to mid-secretory phase (LH+7), in women with unexplained infertility.Methods2-D gel electrophoresis was performed to analyze the proteomic changes between early- (n = 8) and mid-secretory (n = 8) phase endometrium of women with unexplained infertility. The differentially expressed protein spots were identified by LC-MS analysis and validated by immunoblotting and immuno-histochemical analysis in early- (n = 4) and mid-secretory (n = 4) phase endometrium of infertile women. Validated proteins were also analyzed in early- (n = 4) and mid-secretory (n = 4) phase endometrium of fertile women.ResultsNine proteins were found to be differentially expressed between early- and mid- secretory phases of endometrium of infertile women. The expression of Ras-related protein Rap-1b, Protein disulfide isomerase A3, Apolipoprotein-A1 (Apo-A1), Cofilin-1 and RAN GTP-binding nuclear protein (Ran) were found to be significantly increased, whereas, Tubulin polymerization promoting protein family member 3, Superoxide dismutase [Cu-Zn], Sorcin, and Proteasome subunit alpha type-5 were significantly decreased in mid- secretory phase endometrium of infertile women as compared to early-secretory phase endometrium of infertile women. Validation of 4 proteins viz. Sorcin, Cofilin-1, Apo-A1 and Ran were performed in separate endometrial biopsy samples from infertile women. The up-regulated expression of Sorcin and down-regulated expression of Cofilin-1 and Apolipoprotein-A1, were observed in mid-secretory phase as compared to early-secretory phase in case of fertile women.ConclusionsDe-regulation of the expression of Sorcin, Cofilin-1, Apo-A1 and Ran, during early- to mid-secretory phase may have physiological significance and it may be one of the causes for altered differentiation and/or maturation of endometrium, in women with unexplained infertility.

Dataset Information

The mid-secretory endometrial transcriptomic landscape in endometriosis: a meta-analysis.

Publications

The mid-secretory endometrial transcriptomic landscape in endometriosis: a meta-analysis.

Similar Datasets

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