Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum.
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ABSTRACT:
SUBMITTER: Durand R
PROVIDER: S-EPMC90225 | biostudies-literature | 2000 Dec
REPOSITORIES: biostudies-literature
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Use of molecular beacons to detect an antifolate resistance-associated mutation in Plasmodium falciparum.
Durand R R Eslahpazire J J Jafari S S Delabre J F JF Marmorat-Khuong A A di Piazza J P JP Le Bras J J
Antimicrobial agents and chemotherapy 20001201 12
A PCR-based technique using new fluorescent probes, called molecular beacons, was developed to detect the antifolate resistance-associated S108N point mutation in Plasmodium falciparum. One hundred African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This new molecular technique appears to be a promising tool for epidemiological studies. ...[more]
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