Dataset Information

Draft Genome Sequence of Polaromonas eurypsychrophila AER18D-145, Isolated from a Uranium Tailings Management Facility in Northern Saskatchewan, Canada.

ABSTRACT: The 4.8-Mbp draft genome sequence of Polaromonas eurypsychrophila AER18D-145, isolated from a uranium tailings management facility, is reported. The sequence may provide insights into the mechanisms of the hypertolerance of this strain to extreme conditions and help determine its potential for bioremediation applications.

SUBMITTER: Grigoryan AA

PROVIDER: S-EPMC9022503 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Draft Genome Sequence of Polaromonas eurypsychrophila AER18D-145, Isolated from a Uranium Tailings Management Facility in Northern Saskatchewan, Canada.

Grigoryan Alexander A AA Bondici Viorica F VF Kryachko Yuriy Y Khan Nurul H NH Lawrence John R JR Wolfaardt Gideon M GM Withana Gamage Niradha N Shetty Deeksha D Korber Darren R DR

Microbiology resource announcements 20220309 4

The 4.8-Mbp draft genome sequence of Polaromonas eurypsychrophila AER18D-145, isolated from a uranium tailings management facility, is reported. The sequence may provide insights into the mechanisms of the hypertolerance of this strain to extreme conditions and help determine its potential for bioremediation applications. ...[more]

PMID: 35262382

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Project description:Tailings facility failures represent a significant risk to the environment and communities globally, but until now little data was available on the global distribution of risks and characteristics of facilities to ensure proper governance. We conducted a survey and compiled a database with information on tailings facilities disclosed by extractive companies at the request of institutional investors. Despite limitations in the data, this information disclosure request represents the most comprehensive survey of tailings facilities ever undertaken. The compiled dataset includes 1743 tailings facilities and provides insights into a range of topics including construction method, stability, consequence of failure, stored volume, and the rate of uptake of alternative technologies to dewater tailings and reduce geotechnical risk. Our analysis reveals that 10 per cent of tailings facilities reported notable stability concerns or failure to be confirmed or certified as stable at some point in their history, with distinct trends according to construction method, governance, age, height, volume and seismic hazard. Controversy has surrounded the safety of tailings facilities, most notably upstream facilities, for many years but in the absence of definitive empirical data differentiating the risks of different facility types, upstream facilities have continued to be used widely by the industry and a consensus has emerged that upstream facilities can theoretically be built safely under the right circumstances. Our findings reveal that in practice active upstream facilities report a higher incidence of stability issues (18.3%) than other facility types, and that this elevated risk persists even when these facilities are built in high governance settings. In-pit/natural landform and dry-stack facilities report lower incidence of stability issues, though the rate of stability issues is significant by engineering standards (> 2 per cent) across all construction methods, highlighting the universal importance of careful facility management and governance. The insights reported here can assist the global governance of tailings facility stability risks.

Project description:The extraction and processing of uranium (U) have polluted large areas worldwide, rendering anthropogenic extreme environments inhospitable to most species. Noticeably, these sites are of great interest for taxonomical and applied bioprospection of extremotolerant species successfully adapted to U tailings contamination. As an example, in this work we have studied a microalgae species that inhabits extreme U tailings ponds at the Saelices mining site (Salamanca, Spain), characterized as acidic (pH between 3 and 4), radioactive (around 4 μSv h-1) and contaminated with metals, mainly U (from 25 to 48 mg L-1) and zinc (from 17 to 87 mg L-1). After isolation of the extremotolerant ChlSP strain, morphological characterization and internal transcribed spacer (ITS)-5.8S gene sequences placed it in the Chlamydomonadaceae, but BLAST analyses identity values, against the nucleotide datasets at the NCBI database, were very low (<92%). We subjected the ChlSP strain to an artificial selection protocol to increase the U uptake and investigated its response to selection. The ancestral strain ChlSP showed a U-uptake capacity of ≈4.30 mg U g-1 of dry biomass (DB). However, the artificially selected strain ChlSG was able to take up a total of ≈6.34 mg U g-1 DB, close to the theoretical maximum response (≈7.9 mg U g-1 DB). The selected ChlSG strain showed two possible U-uptake mechanisms: the greatest proportion by biosorption onto cell walls (ca. 90%), and only a very small quantity, ~0.46 mg g-1 DB, irreversibly bound by bioaccumulation. Additionally, the kinetics of the U-uptake process were characterized during a microalgae growth curve; ChlSG cells removed close to 4 mg L-1 of U in 24 days. These findings open up promising prospects for sustainable management of U tailings waters based on newly evolved extremotolerants and outline the potential of artificial selection in the improvement of desired features in microalgae by experimental adaptation and selection.

Dataset Information

Draft Genome Sequence of Polaromonas eurypsychrophila AER18D-145, Isolated from a Uranium Tailings Management Facility in Northern Saskatchewan, Canada.

Publications

Draft Genome Sequence of Polaromonas eurypsychrophila AER18D-145, Isolated from a Uranium Tailings Management Facility in Northern Saskatchewan, Canada.

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