Dataset Information

Complete Coding Genome Sequence of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolated from Penaeus (Litopenaeus) vannamei Shrimp in Ecuador.

ABSTRACT: Infectious hypodermal and hematopoietic necrosis virus (IHHNV), recognized as Penaeus stylirostris penstyldensovirus 1 (PstDV1), has been associated with the runt-deformity syndrome (RDS) in cultured Penaeus (Litopenaeus) vannamei and Penaeus monodon shrimp. Here, we report the second published genome sequence of IHHNV, which was isolated from P. vannamei shrimp cultured in Ecuador.

SUBMITTER: Bajana L

PROVIDER: S-EPMC9022545 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Complete Coding Genome Sequence of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolated from Penaeus (Litopenaeus) vannamei Shrimp in Ecuador.

Bajaña Leandro L Betancourt Irma I Bayot Bonny B

Microbiology resource announcements 20220317 4

Infectious hypodermal and hematopoietic necrosis virus (IHHNV), recognized as Penaeus stylirostris penstyldensovirus 1 (PstDV1), has been associated with the runt-deformity syndrome (RDS) in cultured Penaeus (Litopenaeus) vannamei and Penaeus monodon shrimp. Here, we report the second published genome sequence of IHHNV, which was isolated from P. vannamei shrimp cultured in Ecuador. ...[more]

PMID: 35297684

Similar Datasets

Project description:BackgroundA virus-like particle (VLP) is an excellent tool for a compound delivery system due to its simple composition, symmetrical structure and self-assembly. Its surface modification both chemically and genetically is established, leading to the target-specific delivery and improved encapsulation efficiency. However, its physical stabilities against many harsh conditions that guarantee long term storage and oral administration have been much less studied.MethodsIHHNV-VLPs were reconstructed from recombinant IHHNV capsid protein in E. coli. Their physical properties against three strong physical conditions including long term storage (0-30 days) in 4 °C, physical stabilities against broad ranged pH (4-9) and against three types of digestive enzymes were tested. Disassembly and reassembly of VLPs for encapsidating an enhanced green fluorescent protein tagged plasmid DNA (EGFP-VLPs) were controlled by the use of reducing agent (DTT) and calcium specific chelating agent (EGTA). Lastly, delivering ability of EGFP-VLPs was performed in vivo by intramuscular injection and traced the expression of GFP in the shrimp tissues 24 hr post-injection.ResultsUpon its purification, IHHNV-VLPs were able to be kept at 4 °C up to 30 days with only slight degradation. They were very stable in basic condition (pH 8-9) and to a lesser extent in acidic condition (pH 4-6) while they could stand digestions of trypsin and chymotrypsin better than pepsin. As similar with many other non-enveloped viruses, the assembly of IHHNV-VLPs was dependent on both disulfide bridging and calcium ions which allowed us to control disassembly and reassembly of these VLPs to pack EGFP plasmid DNA. IHHNV-VLPs could deliver EGFP plasmids into shrimp muscles and gills as evident by RT-PCR and confocal microscopy demonstrating the expression of GFP in the targeted tissues.DiscussionThere are extensive data in which capsid proteins of the non-enveloped viruses in the form of VLPs are constructed and used as nano-containers for therapeutic compound delivery. However, the bottleneck of its application as an excellent delivery container for oral administration would rely solely on physical stability and interacting ability of VLPs to the host cells. These properties are retained for IHHNV-VLPs reported herein. Thus, IHHNV-VLPs would stand as a good applicable nanocontainer to carry therapeutic agents towards the targeting tissues against ionic and digestive conditions via oral administration in aquaculture field.

Dataset Information

Complete Coding Genome Sequence of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolated from Penaeus (Litopenaeus) vannamei Shrimp in Ecuador.

Publications

Complete Coding Genome Sequence of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolated from <i>Penaeus</i> (<i>Litopenaeus</i>) <i>vannamei</i> Shrimp in Ecuador.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets