Unknown

Dataset Information

0

Characterization of Class 1 integrons from Pseudomonas aeruginosa that contain the bla(VIM-2) carbapenem-hydrolyzing beta-lactamase gene and of two novel aminoglycoside resistance gene cassettes.


ABSTRACT: Two clonally unrelated Pseudomonas aeruginosa clinical strains, RON-1 and RON-2, were isolated in 1997 and 1998 from patients hospitalized in a suburb of Paris, France. Both isolates expressed the class B carbapenem-hydrolyzing beta-lactamase VIM-2 previously identified in Marseilles in the French Riviera. In both isolates, the bla(VIM-2) cassette was part of a class 1 integron that also encoded aminoglycoside-modifying enzymes. In one case, two novel aminoglycoside resistance gene cassettes, aacA29a and aacA29b, were located at the 5' and 3' end of the bla(VIM-2) gene cassette, respectively. The aacA29a and aacA29b gene cassettes were fused upstream with a 101-bp part of the 5' end of the qacE cassette. The deduced amino acid sequence AAC(6')-29a protein shared 96% identity with AAC(6')-29b but only 34% identity with the aacA7-encoded AAC(6')-I1, the closest relative of the AAC(6')-I family enzymes. These aminoglycoside acetyltransferases had amino acid sequences much shorter (131 amino acids) than the other AAC(6')-I enzymes (144 to 153 amino acids). They conferred resistance to amikacin, isepamicin, kanamycin, and tobramycin but not to gentamicin, netilmicin, and sisomicin.

SUBMITTER: Poirel L 

PROVIDER: S-EPMC90325 | biostudies-literature | 2001 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Characterization of Class 1 integrons from Pseudomonas aeruginosa that contain the bla(VIM-2) carbapenem-hydrolyzing beta-lactamase gene and of two novel aminoglycoside resistance gene cassettes.

Poirel L L   Lambert T T   Türkoglü S S   Ronco E E   Gaillard J J   Nordmann P P  

Antimicrobial agents and chemotherapy 20010201 2


Two clonally unrelated Pseudomonas aeruginosa clinical strains, RON-1 and RON-2, were isolated in 1997 and 1998 from patients hospitalized in a suburb of Paris, France. Both isolates expressed the class B carbapenem-hydrolyzing beta-lactamase VIM-2 previously identified in Marseilles in the French Riviera. In both isolates, the bla(VIM-2) cassette was part of a class 1 integron that also encoded aminoglycoside-modifying enzymes. In one case, two novel aminoglycoside resistance gene cassettes, aa  ...[more]

Similar Datasets

| S-EPMC132756 | biostudies-literature
| S-EPMC90765 | biostudies-literature
| S-EPMC90711 | biostudies-literature
| S-EPMC127086 | biostudies-literature
| S-EPMC90451 | biostudies-literature
| S-EPMC296202 | biostudies-literature
| S-EPMC3019676 | biostudies-literature
| S-EPMC90438 | biostudies-literature
| S-EPMC3020596 | biostudies-literature
| S-EPMC2043176 | biostudies-literature