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Zonula occludens-1 expression is reduced in nasal epithelial cells of allergic rhinitis patients.


ABSTRACT: Allergic rhinitis (AR) is a common allergic disease characterized by disruption of nasal epithelial barrier. In this study, we investigated the mRNA expression of zonula occludens-1 (ZO-1), ZO-2 and ZO-3 and histone deacetylase 1 (HDAC1) and HDAC2 in AR patients compared to healthy controls. RNA samples were extracted from nasal epithelial cells of house dust mites (HDMs)-sensitized AR patients and healthy controls (n = 28 in each group). The RNAs were reverse transcribed into cDNAs for measurement of ZO-1, ZO-2, ZO-3, HDAC1 and HDAC2 expression levels by quantitative PCR. The mRNA expression of ZO-1 was significantly decreased in AR patients compared to healthy controls (p = 0.010). No significant difference was observed in the expression levels of ZO-2, ZO-3, HDAC1 and HDAC2 in AR patients compared to healthy controls. We found significant associations of higher HDAC2 levels in AR patients with lower frequency of changing bedsheet (p = 0.043) and with AR patients sensitized to Dermatophagoides farinae (p = 0.041). Higher expression of ZO-2 was observed in AR patients who had pets (p = 0.007). In conclusion, our data indicated that ZO-1 expression was lower in AR patients contributing to decreased integrity of nasal epithelial barrier integrity, and HDAC2 may be involved in the pathogenesis of the disease.

SUBMITTER: Siti Sarah CO 

PROVIDER: S-EPMC9037125 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Zonula occludens-1 expression is reduced in nasal epithelial cells of allergic rhinitis patients.

Siti Sarah Che Othman CO   Nur Husna Siti Muhamad SM   Md Shukri Norasnieda N   Wong Kah Keng KK   Mohd Ashari Noor Suryani NS  

PeerJ 20220422


Allergic rhinitis (AR) is a common allergic disease characterized by disruption of nasal epithelial barrier. In this study, we investigated the mRNA expression of zonula occludens-1 (<i>ZO-1</i>), <i>ZO-2</i> and <i>ZO-3</i> and histone deacetylase 1 (<i>HDAC1</i>) and <i>HDAC2</i> in AR patients compared to healthy controls. RNA samples were extracted from nasal epithelial cells of house dust mites (HDMs)-sensitized AR patients and healthy controls (<i>n</i> = 28 in each group). The RNAs were r  ...[more]

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