Quantifying EGFR endosomal recycling via immunofluorescence in breast cancer cells.
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ABSTRACT:
SUBMITTER: Lonic A
PROVIDER: S-EPMC9038772 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
Quantifying EGFR endosomal recycling via immunofluorescence in breast cancer cells.
Lonic Ana A Onglao Winona W Khew-Goodall Yeesim Y
STAR protocols 20220413 2
Previously published protocols for quantification of endosomal recycling are limited by the use of radioactive reagents, washing of cells in reducing buffers, or the requirement for large numbers of cells. Here, we describe a protocol for quantification of endosomal recycling using immunofluorescence that is optimized for EGFR in BT-549 breast cancer cells but could be applied to other RTKs and cell lines. Our protocol enables quick assessment of recycling and uses a relatively small number of c ...[more]
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