An in vivo orthotopic serial passaging model for a metastatic renal cancer study.
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ABSTRACT:
SUBMITTER: Nishida J
PROVIDER: S-EPMC9043777 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
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An <i>in vivo</i> orthotopic serial passaging model for a metastatic renal cancer study.
Nishida Jun J Miyakuni Kosuke K Miyazono Kohei K Ehata Shogo S
STAR protocols 20220418 2
We developed an <i>in vivo</i> serial passaging model for renal cancer with orthotopic renal subcapsular inoculation. We detail the procedures for renal subcapsular inoculation of cancer cells in mice, followed by <i>in vivo</i> and <i>ex</i><i>vivo</i> bioluminescence imaging, tumor-bearing kidney dissociation, and <i>in vivo</i> passaging. This protocol is capable of reproducing the coevolution between cancer cells and the primary tumor microenvironment. It enables us to unveil the systemic dy ...[more]
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