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Simple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor.

ABSTRACT: In this study an impedimetric immunosensor was developed in order to determine ciprofloxacin (CIP) in wastewater samples, an emergent contaminant widely found in wastewater. To achieve this, an anti-ciprofloxacin antibody was immobilized on the surface of a printed carbon electrode. Then, the developed immunosensor was applied in wastewater samples from Université Laval residences (Québec, Canada) through the load transfer resistance (R _ct) using [Fe(CN)₆]^3-/4- as a redox probe, and the average CIP concentration was found to be 2.90 × 10^-4 μg mL^-1. The observed R _ct changes presented a linear relationship from CIP concentrations of 10^-5 to 1.0 μg mL^-1, with detection and quantification limits of 2.50 × 10^-6 and 7.90 × 10^-6 μg mL^-1, respectively. The immunosensor presented high selectivity and repeatability, as well as a good recovery rate in wastewater samples (97%). Significant interference with other compounds was not observed. The proposed method requires only 30 μL of sample without the use of organic solvents or preceding sample preparation and/or extraction techniques. Moreover, the method is fast: only 20 min of incubation followed by 2 min of analysis time was sufficient to obtain the CIP concentration. The method's estimated cost is U$ 2.00 per sample.

SUBMITTER: Lamarca RS

PROVIDER: S-EPMC9047983 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Simple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor.

Lamarca Rafaela Silva RS de Faria Ricardo Adriano Dorledo RAD Zanoni Maria Valnice Boldrin MVB Nalin Marcelo M de Lima Gomes Paulo Clairmont Feitosa PCF Messaddeq Younès Y

RSC advances 20200108 4

In this study an impedimetric immunosensor was developed in order to determine ciprofloxacin (CIP) in wastewater samples, an emergent contaminant widely found in wastewater. To achieve this, an anti-ciprofloxacin antibody was immobilized on the surface of a printed carbon electrode. Then, the developed immunosensor was applied in wastewater samples from Université Laval residences (Québec, Canada) through the load transfer resistance (R ct) using [Fe(CN)6]3-/4-< ...[more]

PMID: 35494561

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Project description:Effective removal of organic and inorganic impurities by adsorption technique requires the preparation of new materials characterized by low production costs, significant sorption capacity, and reduced toxicity, derived from natural and renewable sources. To address these challenges, new adsorbents have been developed in the form of polymer microspheres based on ethylene glycol dimethacrylate (EGDMA) and vinyl acetate (VA) (EGDMA/VA) containing starch (St) modified with boric acid (B) and dodecyl-S-thiuronium dodecylthioacetate (DiTDTA) for the removal of dyes: C.I. Basic Blue 3 (BB3) and C.I. Acid Green 16 (AG16) and heavy metal ions (M(II)): Cu(II), Ni(II), and Zn(II) from water and wastewater. The adsorbents were characterized by ATR/FT-IR, DSC, SEM, BET, EDS, and pHPZC methods. These analyses demonstrated the successful modification of microspheres and the increased thermal resistance resulting from the addition of the modified starch. The point of zero charge for EGDMA/VA was 7.75, and this value decreased with the addition of modified starch (pHPZC = 6.62 for EGDMA/VA-St/B and pHPZC = 5.42 for EGDMA/VA-St/DiTDTA). The largest specific surface areas (SBET) were observed for the EGDMA/VA microspheres (207 m2/g), and SBET value slightly decreases with the modified starch addition (184 and 169 m2/g) as a consquence of the pores stopping by the big starch molecules. The total pore volumes (Vtot) were found to be in the range from 0.227 to 0.233 cm3/g. These materials can be classified as mesoporous, with an average pore diameter (W) of approximately 55 Å (5.35-6.10 nm). The SEM and EDS analyses indicated that the EGDMA/VA microspheres are globular in shape with well-defined edges and contain 73.06% of carbon and 26.94% of oxygen. The microspheres containing modified starch exhibited a loss of smoothness with more irregular shape. The adsorption efficiency of dyes and heavy metal ions depends on the phases contact time, initial adsorbate concentration and the presence of competing electrolytes and surfactants. The equilibrium data were better fitted by the Freundlich isotherm model than by the Langmuir, Temkin, and Dubinin-Radushkevich models. The highest experimental adsorption capacities were observed for the BB3 dye which were equal to 193 mg/g, 190 mg/g, and 194 mg/g for EGDMA/VA, EGDMA/VA-St/B, EGDMA/VA-St/DiTDTA, respectively. The dyes and heavy metal ions were removed very rapidly and the time required to reach system equilibrium was below 20 min for M(II), 40 min for BB3, and 120 min for AG16. 50% v/v methanol and its mixture with 1 M HCl and NaCl for dyes and 1 M HCl for M(II) desorbed these impurities efficiently.

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Simple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor.

Publications

Simple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor.

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