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Development and evaluation of an automated multi-channel multiplug filtration cleanup device for pesticide residue analysis on mulberry leaves and processed tea.

ABSTRACT: An automated multi-channel multiplug filtration cleanup (m-PFC) device was designed and developed. m-PFC columns were suitably installed in the device. The cycle times, speed and nitrogen pressure parameters of the m-PFC column were optimized. The device was utilized to analyze the 82 pesticide residues in fresh mulberry leaves and processed tea with GC-MS/MS detection. Method validation was performed on 82 pesticide residues in fresh mulberry leaves and processed tea at spiked levels of 0.01, 0.05 and 0.5 mg kg^-1. The fortified recoveries of 82 pesticides were 72-115% and the relative standard deviations were 1-15%, except for diniconazole and clodinafop-propargyl in mulberry leaves. The automated multi-channel m-PFC device was successfully applied to detect the pesticide residues in fresh mulberry leaves and processed tea samples. With comparison to the conventional QuEChERS method, the current method using this device did not need additional vortex or centrifugation steps, and could process 48-64 samples in about one hour. The automated m-PFC method saved labor and improved the precision and was shown to be efficient and practical in pesticide residue analysis.

SUBMITTER: Wu Y

PROVIDER: S-EPMC9048779 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Development and evaluation of an automated multi-channel multiplug filtration cleanup device for pesticide residue analysis on mulberry leaves and processed tea.

Wu Yangliu Y An Quanshun Q Wu Jun J Li Ping P He Jianhong J Pan Canping C

RSC advances 20200114 5

An automated multi-channel multiplug filtration cleanup (m-PFC) device was designed and developed. m-PFC columns were suitably installed in the device. The cycle times, speed and nitrogen pressure parameters of the m-PFC column were optimized. The device was utilized to analyze the 82 pesticide residues in fresh mulberry leaves and processed tea with GC-MS/MS detection. Method validation was performed on 82 pesticide residues in fresh mulberry leaves and processed tea at spiked levels of 0.01, 0 ...[more]

PMID: 35496093

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Project description:ObjectiveMulberry (Morus spp.) fruits and leaves have been proven to possess nutraceutical properties. Due to its fast and easy growing characteristics, mulberry fruits (MF) and leaves (ML) potentially emerge as a great source of functional foods. This study aims to enhance bioactivities (antioxidant, anti-inflammation, and hypoglycemic activity) of MF and ML via submerged fermentation using bacteria (Lactobacillus plantarum TAR 4), yeast (Baker's yeast and red yeast) and fungi (Tempeh and Tapai starter).MethodsIn this study, 25% (mass to volume ratio) of MF and ML were fermented (48 h) with 1% (mass to volume ratio) of different microbial cultures, respectively. Effects of different fermentations on MF and ML were determined based on the changes of total phenolics (TPC), flavonoids (TFC), anthocyanins, total sugar, DPPH activity, ferric reducing antioxidant power (FRAP), albumin denaturation inhibition activity (ADI), anti-lipoxygenase activity and α-amylase inhibition activity (AI).ResultsGenerally, ML had higher AI than MF. However, MF exhibited higher DPPH, FRAP and anti-lipoxygenase activity than ML. After all forms of fermentation, DPPH and AI activity of MF and ML were increased significantly (P < 0.05). However, the effects of fermentation on TPC, FRAP, ADI and anti-lipoxygenase activity of MF were in contrast with ML. TPC, FRAP and anti-lipoxygenase activity of ML were enhanced, but reduced in MF after fermentation. Although the effects exerted by different microorganisms in MF and ML fermentation were different, the bioactivities of MF and ML were generally improved after fermentation. Fermentation by Tempeh starter enhanced TPC (by 2-fold), FRAP (by 2.3-fold), AI (at 10% increment) and anti-lipoxygenase activity (by 5-fold) of ML, whereas Tapai fermentation effectively enhanced the DPPH (at 17% increment) and ADI (by 2-fold) activity of MF.ConclusionFindings of this study provide an insight into the future process design of MF and ML processing into novel functional foods.

Project description:Mulberry (Morus alba L.) leaves have long been considered beneficial in traditional Chinese medicine to treat infectious and internal diseases. Recently studies have discovered that the mulberry leaf's total flavonoids (MLF) display excellent hypoglycemia properties. However, the active ingredients and their molecular mechanisms are still uncharacterized. In this study, we explored the hypoglycemic effects of MLF and mulberry leaf polysaccharides (MLP) on ob/ob mice, an animal model of type 2 diabetes mellitus (T2DM), compared with Ramulus Mori (Sangzhi) alkaloid (RMA). Network pharmacology was employed to identify the potential available targets and active compounds of MLF and RMA against hyperglycemia. Molecular docking, an insulin-resistant cell model and qPCR were employed to verify the antidiabetic activity of the critical compounds and the gene expression profiles of the top molecular targets. Here, the results showed that MLF and MLP improved glucose uptake in insulin-resistant hepatocytes. MLF, MLP and RMA alleviated insulin resistance and glucose intolerance in ob/ob mice. Unlike MLF and MLP, RMA administration did not influence the accumulation of intrahepatic lipids. Network pharmacology analysis revealed that morusin, kuwanon C and morusyunnansin L are the main active compounds of MLF and that they amend insulin resistance and glycemia via the PI3K- Akt signaling pathway, lipid and atherosclerosis pathways, and the AGE-RAGE signaling pathway. Moreover, 1-deoxynojirimycin (DNJ), fagomine (FA), and N-methyl-1-deoxynojirimycin are the primary active ingredients of RMA and target carbohydrate metabolism and regulate alpha-glucosidase activity to produce a potent anti-diabetic effect. The molecular docking results indicated that morusin, kuwanon C and morusyunnansin L are the critical bioactive compounds of MLF. They had high affinities with the key targets adenosine A1 receptor (ADORA1), AKT serine/threonine kinase 1 (AKT1), peroxisome proliferator-activated receptor gamma (PPARγ), and glycogen synthase kinase 3 beta (GSK3β), which play crucial roles in the MLF-mediated glucose-lowering effect. Additionally, morusin plays a role in amending insulin resistance of hepatocytes by repressing the expression of the ADORA1 and PPARG genes. Our results shed light on the mechanism behind the glucose-lowering effects of MLF, suggesting that morusin, kuwanon C, and morusyunnansin L might be promising drug leads for the management of T2DM.

Dataset Information

Development and evaluation of an automated multi-channel multiplug filtration cleanup device for pesticide residue analysis on mulberry leaves and processed tea.

Publications

Development and evaluation of an automated multi-channel multiplug filtration cleanup device for pesticide residue analysis on mulberry leaves and processed tea.

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