Bead based facile assay for sensitive quantification of native state green fluorescent protein.
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ABSTRACT:
SUBMITTER: Kim JM
PROVIDER: S-EPMC9051470 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Publications
Bead based facile assay for sensitive quantification of native state green fluorescent protein.
Kim Jung Min JM Seong Baik Lin BL Lim Dong-Kwon DK
RSC advances 20200301 22
A facile method for the quantification of native state protein is strongly required to accurately determine the amount of expressed protein of interest. Here we report a simple bead-based assay, which can sensitively quantify the amount of native state green fluorescent protein using Ni-NTA (nickel-nitrilotriacetic acid)-modified microbead particles. The bead-based method is simple and straightforward to perform and it showed a highly sensitive capability to detect the expressed fluorescent prot ...[more]
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