Ontology highlight
ABSTRACT:
SUBMITTER: Randegger CC
PROVIDER: S-EPMC90538 | biostudies-literature | 2001 Jun
REPOSITORIES: biostudies-literature
Antimicrobial agents and chemotherapy 20010601 6
Extended-spectrum beta-lactamases (ESBLs), e.g., ESBLs of the TEM or SHV type, compromise the efficacies of expanded-spectrum cephalosporins. An SHV non-ESBL that hydrolyzes only narrow-spectrum cephalosporins can be converted into an SHV ESBL through substitutions at three amino acid positions, 179, 238, or 238--240. In order to improve detection of SHV ESBLs, a novel method, based on real-time PCR monitored with fluorescently labeled hybridization probes and followed by melting curve analysis, ...[more]