Observation of glucose-6-phosphate anomeric exchange in real-time using dDNP hyperpolarised NMR.
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ABSTRACT:
SUBMITTER: Uppala S
PROVIDER: S-EPMC9057779 | biostudies-literature | 2020 Nov
REPOSITORIES: biostudies-literature
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Observation of glucose-6-phosphate anomeric exchange in real-time using dDNP hyperpolarised NMR.
Uppala Sivaranjan S Gamliel Ayelet A Sapir Gal G Sosna Jacob J Gomori J Moshe JM Katz-Brull Rachel R
RSC advances 20201111 67
A hyperpolarised-NMR acquisition approach that is sensitive to the process of glucose-6-phosphate anomerization is presented. Using selective depolarisation of one of the anomer's signals, it is possible to observe the replenishing of this signal due to the fast anomeric exchange of this compound. The forward to reverse reaction rate constants ratio was <i>ca.</i> 1.6. ...[more]
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