Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead.
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ABSTRACT:
SUBMITTER: Chrenek J
PROVIDER: S-EPMC9059157 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
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Protocol for printing 3D neural tissues using the BIO X equipped with a pneumatic printhead.
Chrenek Josie J Kirsch Rebecca R Scheck Kali K Willerth Stephanie M SM
STAR protocols 20220422 2
3D bioprinting-a type of additive manufacturing-can create 3D tissue constructs resembling <i>in vivo</i> tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences' fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be applied to printing 3D tissue models using a variety of cell lines and any chemically crosslinked bioink. These 3D-printed tissue models can be used for applications such as drug screening and dise ...[more]
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