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The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication.

ABSTRACT: To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck embryo fibroblasts (DEFs). Finally, CHv-BAC-ΔUL14 exhibited a significant viral growth defect. Taken together, our findings suggest that DPV UL14 protein regulates viral morphogenesis for efficient viral replication.

SUBMITTER: Wan J

PROVIDER: S-EPMC9062478 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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The protein encoded by the duck plague virus UL14 gene regulates virion morphogenesis and affects viral replication.

Wan Jieyu J Li Fangjie F Wang Mingshu M Cheng Anchun A Tian Bin B Yang Qiao Q Wu Ying Y Ou Xumin X Mao Sai S Sun Di D Zhang Shaqiu S Huang Juan J Gao Qun Q Zhao XinXin X Chen Shun S Liu Mafeng M Jia Renyong R Zhu Dekang D

Poultry science 20220317 6

To investigate the pivotal roles of the duck plague virus (DPV) tegument protein UL14 in viral replication, we generated 2 mutated viruses of DPV by using the bacterial artifcial chromosome system, the UL14-null mutant virus (CHv-BAC-ΔUL14) and the corresponding revertant virus (CHv-BAC-ΔUL14R). We found that the CHv-BAC-ΔUL14 viruses exhibited impaired virion morphogenesis in transmission electron microscopy (TEM) studies. Furthermore, CHv-BAC-ΔUL14 exhibited a plaque size reduction in duck emb ...[more]

PMID: 35489250

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