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A ratiometric and colorimetric probe for detecting Hg²⁺ based on naphthalimide-rhodamine and its staining function in cell imaging.

ABSTRACT: In this work, a rhodamine derivative was developed as a colorimetric and ratiometric fluorescent probe for Hg²⁺. It exhibited a highly sensitive fluorescence response toward Hg²⁺. Importantly, studies revealed that the probe could be used for ratiometric detection of Hg²⁺, with a low detection limit of 0.679 μM. The mechanism of Hg²⁺ detection using compound 1 was confirmed by ESI-MS, ¹H NMR, and HPLC. Upon the addition of Hg²⁺, the rhodamine receptor was induced to be in the ring-opening form via an Hg²⁺-promoted hydrolysis of rhodamine hydrazide to rhodamine acid. In addition to Hg²⁺ detection, the naphthalimide-rhodamine compound was proven to be effective in cell imaging.

SUBMITTER: Wang Y

PROVIDER: S-EPMC9063400 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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A ratiometric and colorimetric probe for detecting Hg2+ based on naphthalimide-rhodamine and its staining function in cell imaging.

Wang Yuesong Y Ding Haichang H Wang Shuai S Fan Congbin C Tu Yayi Y Liu Gang G Pu Shouzhi S

RSC advances 20190415 21

In this work, a rhodamine derivative was developed as a colorimetric and ratiometric fluorescent probe for Hg2+. It exhibited a highly sensitive fluorescence response toward Hg2+. Importantly, studies revealed that the probe could be used for ratiometric detection of Hg2+, with a low detection limit of 0.679 μM. The mechanism of Hg2+ detection using compound 1 was confirmed by ESI-MS, 1H NMR, and HPLC. Upon the addition of Hg2+, t ...[more]

PMID: 35516999

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