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Insight into the DNA adsorption on nitrogen-doped positive carbon dots.

ABSTRACT: Considerable biosensors have been fabricated on the basis of DNA interaction with carbon nanomaterials, such as graphene oxide (GO) nanosheets. Few studies have focused on the rational design of sensors between carbon dots (CDs) and DNA due to the limited understanding of the real forces behind the adsorption of DNA on CDs. In this work, nitrogen doping-positive CDs (N-CDs), which can quench fluorophore-labeled DNA, were investigated to ascertain the interaction between the CDs and DNA. With reference to DNA adsorption on GO, the adsorption capacity and kinetics of N-CDs for DNA were studied. Desorption of DNA from these surfaces was also measured. Moreover, DNA desorption and anchoring force of N-CDs to DNA were different from those of GO, given that the prepared N-CDs and GO were positively and negatively charged, respectively. Accordingly, DNA was adsorbed on N-CDs mainly via electrostatic adsorption and other forces, such as nucleobase effect, hydrophobic interaction, and van der Waals (vdW) forces. This study enhanced the basic knowledge of DNA adsorption on some CDs for further study in the application of CDs in bioanalysis or biomedicine.

SUBMITTER: Li F

PROVIDER: S-EPMC9063714 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Insight into the DNA adsorption on nitrogen-doped positive carbon dots.

Li Fenglan F Cai Qianqian Q Hao Xiaoli X Zhao Chengfei C Huang Zhengjun Z Zheng Yanjie Y Lin Xinhua X Weng Shaohuang S

RSC advances 20190423 22

Considerable biosensors have been fabricated on the basis of DNA interaction with carbon nanomaterials, such as graphene oxide (GO) nanosheets. Few studies have focused on the rational design of sensors between carbon dots (CDs) and DNA due to the limited understanding of the real forces behind the adsorption of DNA on CDs. In this work, nitrogen doping-positive CDs (N-CDs), which can quench fluorophore-labeled DNA, were investigated to ascertain the interaction between the CDs and DNA. With ref ...[more]

PMID: 35515841

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Project description:The current research mainly focuses on transforming low-quality waste into value-added nanomaterials and investigating various ways of utilising them. The hydrothermal preparation of highly fluorescent N-doped carbon dots (N-CDs) was obtained from the carboxymethylcellulose (CMC) of oil palm empty fruit bunches and linear-structured polyethyleneimines (LPEI). Transmission electron microscopy (TEM) analysis showed that the obtained N-CDs had an average size of 3.4 nm. The N-CDs were monodispersed in aqueous solution and were strongly fluorescent under the irradiation of ultra-violet light. A detailed description of the morphology and shape was established using Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). It was shown that LPEI were successfully tuned the fluorescence (PL) properties of CDs in both the intrinsic and surface electronic structures, and enhanced the quantum yield (QY) up to 44%. The obtained N-CDs exhibited remarkable PL stability, long lifetime and pH-dependence behaviour, with the excitation/emission maxima of 350/465.5 nm. Impressively, PL enhancement and blue-shifted emission could be seen with the dilution of the original N-CDs solution. The obtained N-CDs were further applied as fluorescent probe for the identification of Cu2+ in aqueous media. The mechanism could be attributed to the particularly high thermodynamic affinity of Cu2+ for the N-chelate groups over the surface of N-CDs and the fast metal-to-ligand binding kinetics. The linear relationship between the relative quenching rate and the concentration of Cu2+ were applied between 1-30 µM, with a detection limit of 0.93 µM. The fluorescent probe was successfully applied for the detection of Cu2+ in real water. Moreover, a solid-state film of N-CDs was prepared in the presence of poly (vinyl alcohol) (PVA) polymer and found to be stable even after 72-h of continuous irradiation to UV-lamp. In contrast to the aqueous N-CDs, the composite film showed only an excitation independent property, with enhanced PL QY of around 47%. Due to the strong and stable emission nature of N-CDs in both aqueous and solid conditions, the obtained N-CDs are ideal for reducing the overall preparation costs and applying them for various biological and environmental applications in the future.

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Insight into the DNA adsorption on nitrogen-doped positive carbon dots.

Publications

Insight into the DNA adsorption on nitrogen-doped positive carbon dots.

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