Dataset Information

An azo-coupling reaction-based surface enhanced resonance Raman scattering approach for ultrasensitive detection of salbutamol.

ABSTRACT: To date, great achievements with GC-MS, HPLC-MS, and fluorescence biosensing techniques have been made to detect illegal additives of salbutamol (SAL) in swine meat. However, these methods are not suitable for rapid on-site screening due to either costly instruments or rather complicated and/or time consuming sample pretreatments. Herein, a simple, rapid and ultrasensitive approach based on an azo-coupling reaction and surface-enhanced resonance Raman scattering (SERRS) is presented. By combining with a magnetic SERS substrate, an indirect detection for SAL, with a LOD of 1.0 × 10^-11 M (2.39 pg mL^-1), was realized. Moreover, a colorimetric method for naked eye detection was successfully carried out for rapid screening of SAL in concentrations higher than 2.09 × 10^-5 M (5 μg mL^-1). In addition, the proposed method was successfully applied for the rapid determination of SAL in real swine meat. The entire process, including pretreatment, coupling reaction and SERRS detection, was performed within 7 min. Moreover, the SERRS fingerprint band being specific to corresponding functional group guarantees the selectivity for the target molecule. Therefore, the proposed strategy in the present study offers a new way to identify trace amounts of analytes, such as SAL as well as other illegal additives in health-related products and food.

SUBMITTER: Yu S

PROVIDER: S-EPMC9078234 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

An azo-coupling reaction-based surface enhanced resonance Raman scattering approach for ultrasensitive detection of salbutamol.

Yu Shihua S Liu Zhigang Z Zhang Jianpo J Li Hongwei H Xu Na N Yuan Xin-Xin XX Wu Yuqing Y

RSC advances 20180101 10

To date, great achievements with GC-MS, HPLC-MS, and fluorescence biosensing techniques have been made to detect illegal additives of salbutamol (SAL) in swine meat. However, these methods are not suitable for rapid on-site screening due to either costly instruments or rather complicated and/or time consuming sample pretreatments. Herein, a simple, rapid and ultrasensitive approach based on an azo-coupling reaction and surface-enhanced resonance Raman scattering (SERRS) is presented. By combinin ...[more]

PMID: 35542390

Similar Datasets

Project description:In this study, a widely used colloid of Creighton AgNPs (ORI, 1-100 nm, mostly ≤ 40 nm, ∼10 μg mL-1) was rapidly manipulated via tangential flow filtration (TFF) for highly reproducible surface-enhanced (resonance) Raman spectroscopy (SE(R)RS) experiments down to the single-molecule (SM) level. The quasi-spherical AgNPs were size-selected, purified, and concentrated in two TFF fractions of a cutoff diameter of ∼40 nm: AgNP ≤ 40 (∼900 μg mL-1) and AgNP ≥ 40 (∼100 μg mL-1). The SE(R)S-based sensing capabilities of the two TFF fractions were then tested under pre-resonance (632.8 nm) and resonance (532.1 nm) excitation conditions for rhodamine 6G (R6G, 10-6-10-15 M). Both TFF isolates, AgNP ≤ 40 and AgNP ≥ 40, were more effective in adsorbing the R6G analyte (≥91%) than the original colloid (≥78%) at submonolayer coverages. Furthermore, the surface enhancement factors (SEF) of the two TFF fractions were markedly superior to those of ORI under all excitation conditions. SERS at 632.8 nm: only AgNP ≥ 40 enabled the detection of R6G at 10-9 M and produced the largest SEF (2.1 × 106). SE(R)RS and SM-SERRS at 532.1 nm: AgNP ≥ 40 gave rise to the largest SEF values (2.5 × 1010) corresponding to the SM regime down to 10-15 M of R6G. Nevertheless, AgNP ≤ 40 compensated for the size-dependence of the electromagnetic enhancements by an increase in the silver concentration, which led to SEF values comparable to those of AgNP ≥ 40 through additional resonance enhancements. TFF resulted into a ∼100-fold increase (AgNP ≤ 40) in the number of negatively charged AgNPs that were available to electrostatically bridge R6G cations and form SERRS "hot-spots" (AgNP-R6G-AgNP) within the focal volume. Evidently, the interplay between AgNP size, AgNP concentration, and excitation wavelength governs the SE(R)RS enhancements. This study demonstrated that TFF can facilitate the ecofriendly isolation of spherical AgNPs of controlled morphological and plasmonic properties for further enhancing their sensing capabilities as SE(R)RS substrates.

Dataset Information

An azo-coupling reaction-based surface enhanced resonance Raman scattering approach for ultrasensitive detection of salbutamol.

Publications

An azo-coupling reaction-based surface enhanced resonance Raman scattering approach for ultrasensitive detection of salbutamol.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets