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Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes.

ABSTRACT: We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence.

SUBMITTER: Lee HJ

PROVIDER: S-EPMC9078427 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes.

Lee Ha Jung HJ Go Gui Han GH Ro Jong Jin JJ Kim Byeang Hyean BH

RSC advances 20180216 14

We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence. ...[more]

PMID: 35539109

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