Dataset Information

Hydrogel microfibers with perfusable folded channels for tissue constructs with folded morphology.

ABSTRACT: Fiber-based materials with microchannels have drawn considerable attention in recent years owing to their ability to mimic intrinsic morphologies of living tissues. Folded morphologies, which are common in vivo, such as in skeletal muscle capillaries and intestine luminal endoderm, play important roles in the achievement of tissue functions. Here, microfibers with folded hollow channels are fabricated. Channel morphologies, such as straight-folded, double-folded and double-helical channels, can be regulated by adjusting flow conditions in the microfluidic devices. To further demonstrate the potential to be used in tissue engineering, intestine and skeletal muscle constructs are fabricated using these microfibers as building blocks. Furthermore, the properties of perfusability, permeability, cytocompatibility and weavability of the microfibers are evaluated. The asymmetric molecular distributions in the microfibers provide promising platforms for the study of nutrient exchange and energy supplement between normal and tortuous tissues. The new features of biofibers and proof-of-concept of tissue constructs with folded morphologies may contribute to the development of regenerative medicine and drug screening in the future.

SUBMITTER: Liu Y

PROVIDER: S-EPMC9081586 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Hydrogel microfibers with perfusable folded channels for tissue constructs with folded morphology.

Liu Yupeng Y Xu Peidi P Liang Zhe Z Xie Ruoxiao R Ding Mingyu M Liu Hongxia H Liang Qionglin Q

RSC advances 20180627 42

Fiber-based materials with microchannels have drawn considerable attention in recent years owing to their ability to mimic intrinsic morphologies of living tissues. Folded morphologies, which are common <i>in vivo</i>, such as in skeletal muscle capillaries and intestine luminal endoderm, play important roles in the achievement of tissue functions. Here, microfibers with folded hollow channels are fabricated. Channel morphologies, such as straight-folded, double-folded and double-helical channel ...[more]

PMID: 35540297

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Project description:Despite the significant technological advancement in tissue engineering, challenges still exist towards the development of complex and fully functional tissue constructs that mimic their natural counterparts. To address these challenges, bioprinting has emerged as an enabling technology to create highly organized three-dimensional (3D) vascular networks within engineered tissue constructs to promote the transport of oxygen, nutrients, and waste products, which can hardly be realized using conventional microfabrication techniques. Here, we report the development of a versatile 3D bioprinting strategy that employs biomimetic biomaterials and an advanced extrusion system to deposit perfusable vascular structures with highly ordered arrangements in a single-step process. In particular, a specially designed cell-responsive bioink consisting of gelatin methacryloyl (GelMA), sodium alginate, and 4-arm poly(ethylene glycol)-tetra-acrylate (PEGTA) was used in combination with a multilayered coaxial extrusion system to achieve direct 3D bioprinting. This blend bioink could be first ionically crosslinked by calcium ions followed by covalent photocrosslinking of GelMA and PEGTA to form stable constructs. The rheological properties of the bioink and the mechanical strengths of the resulting constructs were tuned by the introduction of PEGTA, which facilitated the precise deposition of complex multilayered 3D perfusable hollow tubes. This blend bioink also displayed favorable biological characteristics that supported the spreading and proliferation of encapsulated endothelial and stem cells in the bioprinted constructs, leading to the formation of biologically relevant, highly organized, perfusable vessels. These characteristics make this novel 3D bioprinting technique superior to conventional microfabrication or sacrificial templating approaches for fabrication of the perfusable vasculature. We envision that our advanced bioprinting technology and bioink formulation may also have significant potentials in engineering large-scale vascularized tissue constructs towards applications in organ transplantation and repair.

Project description:Devices for in vitro culture of three-dimensional (3D) skeletal muscle tissues have multiple applications, including tissue engineering and muscle-powered biorobotics. In both cases, it is crucial to recreate a biomimetic environment by using tailored scaffolds at multiple length scales and to administer prodifferentiative biophysical stimuli (e.g., mechanical loading). On the contrary, there is an increasing need to develop flexible biohybrid robotic devices capable of maintaining their functionality beyond laboratory settings. In this study, we describe a stretchable and perfusable device to sustain cell culture and maintenance in a 3D scaffold. The device mimics the structure of a muscle connected to two tendons: Tendon-Muscle-Tendon (TMT). The TMT device is composed of a soft (E ∼ 6 kPa) porous (pore diameter: ∼650 μm) polyurethane scaffold, encased within a compliant silicone membrane to prevent medium evaporation. Two tendon-like hollow channels interface the scaffold with a fluidic circuit and a stretching device. We report an optimized protocol to sustain C2C12 adhesion by coating the scaffold with polydopamine and fibronectin. Then, we show the procedure for the soft scaffold inclusion in the TMT device, demonstrating the device's ability to bear multiple cycles of elongations, simulating a protocol for cell mechanical stimulation. By using computational fluid dynamic simulations, we show that a flow rate of 0.62 mL/min ensures a wall shear stress value safe for cells (<2 Pa) and 50% of scaffold coverage by an optimal fluid velocity. Finally, we demonstrate the effectiveness of the TMT device to sustain cell viability under perfusion for 24 h outside of the CO2 incubator. We believe that the proposed TMT device can be considered an interesting platform to combine several biophysical stimuli, aimed at boosting skeletal muscle tissue differentiation in vitro, opening chances for the development of muscle-powered biohybrid soft robots with long-term operability in real-world environments.

Dataset Information

Hydrogel microfibers with perfusable folded channels for tissue constructs with folded morphology.

Publications

Hydrogel microfibers with perfusable folded channels for tissue constructs with folded morphology.

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