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Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution.

ABSTRACT: In rod-shaped bacteria, morphological plasticity occurs in response to stress, which blocks cell division to promote filamentation. We demonstrate here that overexpression of the patatin-like phospholipase variant CapV_Q329R, but not CapV, causes pronounced sulA-independent pyridoxine-inhibited cell filamentation in the Escherichia coli K-12-derivative MG1655 associated with restriction of flagella production and swimming motility. Conserved amino acids in canonical patatin-like phospholipase A motifs, but not the nucleophilic serine, are required to mediate CapV_Q329R phenotypes. Furthermore, CapV_Q329R production substantially alters the lipidome and colony morphotype including rdar biofilm formation with modulation of the production of the biofilm activator CsgD, and affects additional bacterial traits such as the efficiency of phage infection and antimicrobial susceptibility. Moreover, genetically diverse commensal and pathogenic E. coli strains and Salmonella typhimurium responded with cell filamentation and modulation in colony morphotype formation to CapV_Q329R expression. In conclusion, this work identifies the CapV variant CapV_Q329R as a pleiotropic regulator, emphasizes a scaffold function for patatin-like phospholipases, and highlights the impact of the substitution of a single conserved amino acid for protein functionality and alteration of host physiology.

SUBMITTER: Li F

PROVIDER: S-EPMC9095652 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution.

Li Fengyang F Cao Lianying L Bähre Heike H Kim Soo-Kyoung SK Schroeder Kristen K Jonas Kristina K Koonce Kira K Mekonnen Solomon A SA Mohanty Soumitra S Bai Fengwu F Brauner Annelie A Lee Vincent T VT Rohde Manfred M Römling Ute U

NPJ biofilms and microbiomes 20220511 1

In rod-shaped bacteria, morphological plasticity occurs in response to stress, which blocks cell division to promote filamentation. We demonstrate here that overexpression of the patatin-like phospholipase variant CapV<sub>Q329R</sub>, but not CapV, causes pronounced sulA-independent pyridoxine-inhibited cell filamentation in the Escherichia coli K-12-derivative MG1655 associated with restriction of flagella production and swimming motility. Conserved amino acids in canonical patatin-like phosph ...[more]

PMID: 35546554

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Dataset Information

Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution.

Publications

Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution.

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