Construction of a bioluminescent reporter strain To detect polychlorinated biphenyls
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ABSTRACT:
SUBMITTER: Layton AC
PROVIDER: S-EPMC90961 | biostudies-literature | 1998 Dec
REPOSITORIES: biostudies-literature
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Construction of a bioluminescent reporter strain To detect polychlorinated biphenyls
Layton AC Muccini M Ghosh MM Sayler GS
Applied and environmental microbiology 19981201 12
A bioluminescent reporter strain, Ralstonia eutropha ENV307(pUTK60), was constructed for the detection of polychlorinated biphenyls by inserting the biphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated biphenyls, bioluminescence was induced three- to fourfold over background by biphenyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection limits for these compounds ranged from 0.15 mg/liter ...[more]
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