Dataset Information

Breath volatile metabolome reveals the impact of dietary fibres on the gut microbiota: Proof of concept in healthy volunteers.

ABSTRACT:

Background

Current data suggest that dietary fibre (DF) interaction with the gut microbiota largely contributes to their physiological effects. The bacterial fermentation of DF leads to the production of metabolites, most of them are volatile. This study analyzed the breath volatile metabolites (BVM) profile in healthy individuals (n=15) prior and after a 3-week intervention with chitin-glucan (CG, 4.5 g/day), an insoluble fermentable DF.

Methods

The present exploratory study presents the original data related to the secondary outcomes, notably the analysis of BVM. BVM were analyzed throughout the test days -in fasting state and after standardized meals - using selected ion flow tube mass spectrometry (SIFT-MS). BVM production was correlated to the gut microbiota composition (Illumina sequencing, primary outcome), analyzed before and after the intervention.

Findings

The data reveal that the post-prandial state versus fasting state is a key determinant of BVM fingerprint. Correlation analyses with fecal microbiota spotlighted butyrate-producing bacteria, notably Faecalibacterium, as dominant bacteria involved in butyrate and other BVM expiration. CG intervention promotes interindividual variations of fasting BVM, and decreases or delays the expiration of most exhaled BVM in favor of H₂ expiration, without any consequence on gastrointestinal tolerance.

Interpretation

Assessing BVM is a non-invasive methodology allowing to analyze the influence of DF intervention on the gut microbiota.

Funding

FiberTAG project was initiated from a European Joint Programming Initiative "A Healthy Diet for a Healthy Life" (JPI HDHL) and was supported by the Service Public de Wallonie (SPW-EER, convention 1610365, Belgium).

SUBMITTER: Neyrinck AM

PROVIDER: S-EPMC9108873 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Publications

Breath volatile metabolome reveals the impact of dietary fibres on the gut microbiota: Proof of concept in healthy volunteers.

Neyrinck Audrey M AM Rodriguez Julie J Zhang Zhengxiao Z Nazare Julie-Anne JA Bindels Laure B LB Cani Patrice D PD Maquet Véronique V Laville Martine M Bischoff Stephan C SC Walter Jens J Delzenne Nathalie M NM

EBioMedicine 20220510

<h4>Background</h4>Current data suggest that dietary fibre (DF) interaction with the gut microbiota largely contributes to their physiological effects. The bacterial fermentation of DF leads to the production of metabolites, most of them are volatile. This study analyzed the breath volatile metabolites (BVM) profile in healthy individuals (n=15) prior and after a 3-week intervention with chitin-glucan (CG, 4.5 g/day), an insoluble fermentable DF.<h4>Methods</h4>The present exploratory study pres ...[more]

PMID: 35561452

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Dataset Information

Breath volatile metabolome reveals the impact of dietary fibres on the gut microbiota: Proof of concept in healthy volunteers.

Background

Methods

Findings

Interpretation

Funding

Publications

Breath volatile metabolome reveals the impact of dietary fibres on the gut microbiota: Proof of concept in healthy volunteers.

Similar Datasets

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