Ontology highlight
ABSTRACT:
SUBMITTER: Shepard BD
PROVIDER: S-EPMC91209 | biostudies-literature | 1999 Apr
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 19990401 4
Enterococci have emerged among the leading causes of nosocomial infection. With the goal of analyzing enterococcal genes differentially expressed in environments related to commensal or environmental colonization and infection sites, we adapted and optimized a method more commonly used in the study of eukaryotic gene expression, random arbitrarily primed PCR (RAP-PCR). The RAP-PCR method was systematically optimized, allowing the technique to be used in a highly reproducible manner with gram-pos ...[more]