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Applying selenocysteine-mediated expressed protein ligation to prepare the membrane enzyme selenoprotein S.

ABSTRACT: The intrinsically disordered membrane-bound selenoprotein s (selenos) takes part in the protein quality control pathway, vesicle trafficking, and NF-kB signaling. The reactive selenocysteine (Sec) at the penultimate position is responsible for its enzymatic activity. We report the preparation of the soluble segment as well as the full-length selenos using expressed protein ligation. This chapter discusses the practical considerations of expressed protein ligation using selenopeptides and describes our optimized procedure for the semi-synthesis of selenos.

SUBMITTER: Cheng R

PROVIDER: S-EPMC9126641 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Applying selenocysteine-mediated expressed protein ligation to prepare the membrane enzyme selenoprotein S.

Cheng Rujin R Liu Jun J Daithankar Vidyadhar V Rozovsky Sharon S

Methods in enzymology 20211224

The intrinsically disordered membrane-bound selenoprotein s (selenos) takes part in the protein quality control pathway, vesicle trafficking, and NF-kB signaling. The reactive selenocysteine (Sec) at the penultimate position is responsible for its enzymatic activity. We report the preparation of the soluble segment as well as the full-length selenos using expressed protein ligation. This chapter discusses the practical considerations of expressed protein ligation using selenopeptides and describ ...[more]

PMID: 35101209

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