An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae.
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ABSTRACT:
SUBMITTER: Peng B
PROVIDER: S-EPMC9130285 | biostudies-literature | 2022 May
REPOSITORIES: biostudies-literature
Publications
An in vivo gene amplification system for high level expression in Saccharomyces cerevisiae.
Peng Bingyin B Esquirol Lygie L Lu Zeyu Z Shen Qianyi Q Cheah Li Chen LC Howard Christopher B CB Scott Colin C Trau Matt M Dumsday Geoff G Vickers Claudia E CE
Nature communications 20220524 1
Bottlenecks in metabolic pathways due to insufficient gene expression levels remain a significant problem for industrial bioproduction using microbial cell factories. Increasing gene dosage can overcome these bottlenecks, but current approaches suffer from numerous drawbacks. Here, we describe HapAmp, a method that uses haploinsufficiency as evolutionary force to drive in vivo gene amplification. HapAmp enables efficient, titratable, and stable integration of heterologous gene copies, delivering ...[more]
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