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New Autonomous Water-Enabled Self-Healing Coating Material with Antibacterial-Agent-Releasing Properties.

ABSTRACT: A new autonomous water-enabled self-healing coating with antibacterial-agent-releasing capability was developed for the first time by precipitating an aqueous solution of hydrogen-bonded tannic acid (TA) and polyethylene glycol (PEG) (TA: 5 mg/mL; PEG: 5 mg/mL with M_W = 100 kDa) to form a smooth, uniform coating layer with an average roughness of 0.688 nm and thickness of 22.3 μm on a polymethyl methacrylate (PMMA) substrate after 10 min of incubation. Our method is cost- and time-efficient, as the hydrophilic coating (water contact angle = 65.1°) forms rapidly, binding strongly to the PMMA substrate (adhesive energy = 83 mJ/m²), without the need for pretreatment or surface modification, and is capable of rapid self-repair (approximately 5 min) through hydrogen bonding in aqueous media. Furthermore, adding 0.5 mg/mL of chlorhexidine acetate (CHX), a commonly used antibacterial agent in dentistry, into the TA-PEG emulsion allowed the release of 2.89 μg/mL of the drug from the coating layer, which is promising for actively inhibiting the vitality and growth of bacteria around PMMA dental restorations. The use of CHX-loaded TA-PEG hydrogen-bonded complexes is highly favorable for the fabrication of an autonomous self-healing biocoating with active antibacterial-agent-releasing capability, which can be applied not only in dentistry but also in other medical fields.

SUBMITTER: Kim KH

PROVIDER: S-EPMC9143542 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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New Autonomous Water-Enabled Self-Healing Coating Material with Antibacterial-Agent-Releasing Properties.

Kim Ki-Hak KH Mai Hang-Nga HN Hyun Dong-Choon DC Lee Du-Hyeong DH

Pharmaceutics 20220507 5

A new autonomous water-enabled self-healing coating with antibacterial-agent-releasing capability was developed for the first time by precipitating an aqueous solution of hydrogen-bonded tannic acid (TA) and polyethylene glycol (PEG) (TA: 5 mg/mL; PEG: 5 mg/mL with M<sub>W</sub> = 100 kDa) to form a smooth, uniform coating layer with an average roughness of 0.688 nm and thickness of 22.3 μm on a polymethyl methacrylate (PMMA) substrate after 10 min of incubation. Our method is cost- and time-eff ...[more]

PMID: 35631591

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Project description:ObjectivesSurface pre-reacted glass-ionomer (S-PRG) fillers release antibacterial borate and fluoride ions. We fabricated nanoscale S-PRG fillers (S-PRG nanofillers) for antibacterial coating of tooth surfaces and assessed the antibacterial effects of this coating in vitro. In addition, we creating a canine model of periodontitis to evaluate the effectiveness of S-PRG nanofiller application on tooth roots and improvement of periodontal parameters.MethodsHuman dentin blocks were coated with S-PRG nanofiller (average particle size: 0.48 μm) and then characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectrometer (EDX), and ion-releasing test. Antibacterial effects of dentin blocks coated with S-PRG nanofiller were examined using bacterial strains, Streptococcus mutans and Actinomyces naeslundii. Next, we created an experimental model of periodontitis in furcation of premolars of beagle dogs. Then, S-PRG nanofiller coating was applied onto exposed tooth root surfaces. Periodontal parameters, gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD), and clinical attachment level (CAL), were measured from baseline until 4 weeks. In addition, bone healing was radiographically and histologically examined.ResultsSEM and EDX revealed that S-PRG nanofillers uniformly covered the dentin surface after coating. Dentin blocks coated with S-PRG nanofiller showed ion-releasing property, bacterial growth inhibition, and sterilization effects. In the experimental periodontitis model, S-PRG nanofiller coating significantly reduced clinical inflammatory parameters, such as GI (P < 0.01) and BOP (P < 0.05), compared to uncoated samples. In addition, PPD and CAL significantly decreased by S-PRG nanofiller coating (2 weeks: P < 0.05; 3 and 4 weeks: P < 0.01), suggesting the improvement of periodontitis. Micro-CT and histology revealed that bone healing of furcation defects was enhanced by S-PRG nanofiller coating.ConclusionS-PRG nanofiller coating provides antibacterial effects to tooth surfaces and improves clinical parameters of periodontitis.

Project description:Preferable antibacterial property and osteogenesis are the permanent pursuit for metallic implants. However, it is difficult to satisfy both the properties. In fact, implants may be contaminated with bacteria during storage and surgery, leading to inflammation. Therefore, the antibacterial property of biomaterial surfaces is required not only in the human environment but also at room temperature. In this study, porous structures loaded with a thermosensitive poly (N-isopropylacrylamide) (PNIPAM) hydrogel on a nitinol (NiTi) substrate were constructed. When the temperature is 25 °C, almost all bacteria cannot adhere to the sample surface due to the abundant hydration layer of the PNIPAM hydrogel. Meanwhile, when the temperature is 37 °C, the structure of the PNIPAM hydrogel collapses and the hydration layer disappears due to the temperature change. However, the porous structures lock water in the pores, which results in a high-hydration-rate sample surface. This surface has few bacterial adhesion sites; nevertheless, the adhesion of larger cells to the surface is not impeded by the porous structure. In addition, the PNIPAM hydrogel is soft and biocompatible, so the sample can have better cell adhesion and proliferation than a bare NiTi alloy. Based on these results, it can be concluded that the porous NiTi sample loaded with the thermosensitive PNIPAM hydrogel has the antibacterial property before implantation and the dual function of inhibiting bacterial adhesion and promoting cell adhesion and proliferation after implantation, which shows promising applications in the biomedical field such as orthopedic implantation. Graphical abstract Image 1 Highlights • Nano-porous structures can expand the temperature-sensitive response of PNIPAM by capturing water molecules in the pores.• Porous NiTi loaded with PNIPAM can inhibit the adhesion of bacteria at 25 °C.• Porous NiTi loaded with PNIPAM is resistant to bacterial adhesion but has a positive effect on cells at 37 °C.

Dataset Information

New Autonomous Water-Enabled Self-Healing Coating Material with Antibacterial-Agent-Releasing Properties.

Publications

New Autonomous Water-Enabled Self-Healing Coating Material with Antibacterial-Agent-Releasing Properties.

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