Ontology highlight
ABSTRACT:
SUBMITTER: De Silva NL
PROVIDER: S-EPMC9145043 | biostudies-literature | 2022 May
REPOSITORIES: biostudies-literature
De Silva Nirmitha Lalindi NL De Silva Viraji Nefertiti Hiromel VNH Deerasinghe Arachchige Theja Hemapala ATH Rathnapala Upeksha Lakmini UL Itoh Makoto M Takagi Hidekazu H Weerasooriya Mirani Vasanthamala MV Kato Hirotomo H Yahathugoda Thishan Channa TC
Microorganisms 20220509 5
The recent surge in cutaneous leishmaniasis (CL) in Sri Lanka has rendered clinical diagnosis difficult; thus, laboratory confirmation is indispensable. A modified (two novel inner primers to detect CL caused by Leishmania donovani) nested Internal Transcribed Spacer-1 (ITS1) PCR-Restriction Fragment Length Polymorphism (RFLP) method was developed and tested. The sensitivity of the modified nested PCR was tested using serial dilutions (103 to 10−2) of the DNA extract of a cultured L. donovani DD ...[more]