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The First Homologous Expression System for the β-Lytic Protease of Lysobacter capsici VKM B-2533T, a Promising Antimicrobial Agent.


ABSTRACT: A successful homologous expression system based on Lysobacter capsici VKM B-2533T and the plasmid pBBR1-MCS5 was first developed for a promising bacteriolytic enzyme of this bacterium, β-lytic protease (Blp). In the expression strains, blp gene expression under the regulation of the GroEL(A) and T5 promoters increased by 247- and 667-fold, respectively, as compared with the wild-type strain. After the cultivation of the expression strains L. capsici PGroEL(A)-blp and L. capsici PT5-blp, the Blp yield increased by 6.7- and 8.5-fold, respectively, with respect to the wild-type strain. The cultivation of the expression strain L. capsici PT5-blp was successfully scaled up. Under fermentation conditions the yield of the enzyme increased by 1.6-fold. The developed homologous system was used to express the gene of the bacteriolytic serine protease (Serp) of L. capsici VKM B-2533T. The expression of the serp gene in L. capsici PT5-serp increased by 585-fold. The developed homologous system for the gene expression of bacteriolytic Lysobacter enzymes is potentially biotechnologically valuable, and is promising for creating highly efficient expression strains.

SUBMITTER: Kudryakova I 

PROVIDER: S-EPMC9145596 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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The First Homologous Expression System for the β-Lytic Protease of <i>Lysobacter capsici</i> VKM B-2533<sup>T</sup>, a Promising Antimicrobial Agent.

Kudryakova Irina I   Afoshin Alexey A   Leontyevskaya Elena E   Leontyevskaya Vasilyeva Natalia N  

International journal of molecular sciences 20220520 10


A successful homologous expression system based on <i>Lysobacter capsici</i> VKM B-2533<sup>T</sup> and the plasmid pBBR1-MCS5 was first developed for a promising bacteriolytic enzyme of this bacterium, β-lytic protease (Blp). In the expression strains, <i>blp</i> gene expression under the regulation of the GroEL(A) and T5 promoters increased by 247- and 667-fold, respectively, as compared with the wild-type strain. After the cultivation of the expression strains <i>L. capsici</i> P<sub>GroEL(A)  ...[more]

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