Dataset Information

Molecular characteristics of ESBL-producing Escherichia coli isolated from chickens with colibacillosis.

ABSTRACT:

Background

Avian pathogenic Escherichia coli (APEC) causes colibacillosis, resulting in significant economic losses in the poultry industry.

Objectives

In this study, the molecular characteristics of two extended-spectrum beta-lactamase (ESBL)-producing APEC isolates were compared with previously reported ESBL-producing E. coli isolates.

Methods

The molecular characteristics of E. coli isolates and the genetic environments of the ESBL genes were investigated using whole genome sequencing.

Results

The two ESBL-producing APEC were classified into the phylogenetic groups C and B1 and ST410 and ST162, respectively. Moreover, the ESBL genes of the two isolates were harbored in different Inc plasmids. The EC1809182 strain, harboring the bla_CTX-M-55 gene on the plasmid, exhibited extensive homology to IncFIB (98.4%) and IncFIC(FII) (95.8%). The EC1809191 strain, harboring the bla_CTX-M-1 gene, was homologous to IncI1-I (Gamma) (99.3%). All chromosomes carried the multidrug transporter, mdf(A) gene. Mobile genetic elements, adjacent to CTX-M genes, facilitated the dissemination of genes in the two isolates, analogous to other ESBL-producing E. coli isolates.

Conclusions

This study clarifies the transmission dynamics of CTX-M genes and supports strengthened surveillance to prevent the transmission of the antimicrobial-resistant genes to humans via the food chain.

SUBMITTER: Yoon S

PROVIDER: S-EPMC9149503 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Publications

Molecular characteristics of ESBL-producing <i>Escherichia coli</i> isolated from chickens with colibacillosis.

Yoon Sunghyun S Lee Young Ju YJ

Journal of veterinary science 20220222 3

<h4>Background</h4>Avian pathogenic <i>Escherichia coli</i> (APEC) causes colibacillosis, resulting in significant economic losses in the poultry industry.<h4>Objectives</h4>In this study, the molecular characteristics of two extended-spectrum beta-lactamase (ESBL)-producing APEC isolates were compared with previously reported ESBL-producing <i>E. coli</i> isolates.<h4>Methods</h4>The molecular characteristics of <i>E. coli</i> isolates and the genetic environments of the ESBL genes were investi ...[more]

PMID: 35332711

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