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Isolation, characterization, and avenacin sensitivity of a diverse collection of cereal-root-colonizing fungi.


ABSTRACT: A total of 161 fungal isolates were obtained from the surface-sterilized roots of field-grown oat and wheat plants in order to investigate the nature of the root-colonizing fungi supported by these two cereals. Fungi were initially grouped according to their colony morphologies and then were further characterized by ribosomal DNA sequence analysis. The collection contained a wide range of ascomycetes and also some basidiomycete fungi. The fungi were subsequently assessed for their abilities to tolerate and degrade the antifungal oat root saponin, avenacin A-1. Nearly all the fungi obtained from oat roots were avenacin A-1 resistant, while both avenacin-sensitive and avenacin-resistant fungi were isolated from the roots of the non-saponin-producing cereal, wheat. The majority of the avenacin-resistant fungi were able to degrade avenacin A-1. These experiments suggest that avenacin A-1 is likely to influence the development of fungal communities within (and possibly also around) oat roots.

SUBMITTER: Carter JP 

PROVIDER: S-EPMC91506 | biostudies-literature | 1999 Aug

REPOSITORIES: biostudies-literature

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Isolation, characterization, and avenacin sensitivity of a diverse collection of cereal-root-colonizing fungi.

Carter J P JP   Spink J J   Cannon P F PF   Daniels M J MJ   Osbourn A E AE  

Applied and environmental microbiology 19990801 8


A total of 161 fungal isolates were obtained from the surface-sterilized roots of field-grown oat and wheat plants in order to investigate the nature of the root-colonizing fungi supported by these two cereals. Fungi were initially grouped according to their colony morphologies and then were further characterized by ribosomal DNA sequence analysis. The collection contained a wide range of ascomycetes and also some basidiomycete fungi. The fungi were subsequently assessed for their abilities to t  ...[more]

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