An immuno-enrichment free, validated quantification of tau protein in human CSF by LC-MS/MS.
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ABSTRACT:
SUBMITTER: Self W
PROVIDER: S-EPMC9162344 | biostudies-literature | 2022
REPOSITORIES: biostudies-literature
Publications
An immuno-enrichment free, validated quantification of tau protein in human CSF by LC-MS/MS.
Self Wade W Awwad Khader K Savaryn John Paul JP Schulz Michael M
PloS one 20220602 6
Tau protein is a key target of interest in developing therapeutics for neurodegenerative diseases. Here, we sought to develop a method that quantifies extracellular tau protein concentrations in human cerebrospinal fluid (CSF) without antibody-based enrichment strategies. We demonstrate that the fit-for-purpose validated method in Alzheimer's Disease CSF is limited to quasi quantitative measures of tau surrogate peptides. We also provide evidence that CSF total Tau measures by LC-MS are feasible ...[more]
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