Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry.
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ABSTRACT:
SUBMITTER: Lewandowski TM
PROVIDER: S-EPMC9175107 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
Dual fluorescent labeling of GLP-1R in live cells <i>via</i> enzymatic tagging and bioorthogonal chemistry.
Lewandowski Tracey M TM An Peng P Ramil Carlo P CP Fang Ming M Lin Qing Q
RSC chemical biology 20220517 6
To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells. ...[more]
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