Dataset Information
Expression of mammalian proteins for diagnostics and therapeutics: a review.
Ontology highlight
ABSTRACT: Background
Antibodies have proven to be remarkably successful for biomedical applications. They play important roles in epidemiology and medicine from diagnostics of diseases to therapeutics, treating diseases from incessant chronic diseases such as rheumatology to pandemic outbreaks. With no end in sight for the demand for antibody products, optimizations and new techniques must be expanded to accommodate this.Methods and results
This review discusses optimizations and techniques for antibody production through choice of discovery platforms, expression systems, cell culture mediums, and other strategies to increase expression yield. Each system has its own merits and demerits, and the strategy chosen is critical in addressing various biological aspects.Conclusions
There is still insufficient evidence to validate the efficacy of some of these techniques, and further research is needed to consolidate these industrial production systems. There is no doubt that more strategies, systems, and pipelines will contribute to enhance biopharmaceutical production.
SUBMITTER: Mark JKK
PROVIDER: S-EPMC9175168 | biostudies-literature | 2022 Nov
REPOSITORIES: biostudies-literature
Publications
Expression of mammalian proteins for diagnostics and therapeutics: a review.
Molecular biology reports 20220608 11
<h4>Background</h4>Antibodies have proven to be remarkably successful for biomedical applications. They play important roles in epidemiology and medicine from diagnostics of diseases to therapeutics, treating diseases from incessant chronic diseases such as rheumatology to pandemic outbreaks. With no end in sight for the demand for antibody products, optimizations and new techniques must be expanded to accommodate this.<h4>Methods and results</h4>This review discusses optimizations and technique ...[more]