Ontology highlight
ABSTRACT:
SUBMITTER: Debeche T
PROVIDER: S-EPMC92053 | biostudies-literature | 2000 Apr
REPOSITORIES: biostudies-literature
Debeche T T Cummings N N Connerton I I Debeire P P O'Donohue M J MJ
Applied and environmental microbiology 20000401 4
The gene encoding an alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus D3, AbfD3, was isolated. Characterization of the purified recombinant alpha-L-arabinofuranosidase produced in Escherichia coli revealed that it is highly stable with respect to both temperature (up to 90 degrees C) and pH (stable in the pH range 4 to 12). On the basis of amino acid sequence similarities, this 56, 071-Da enzyme could be assigned to family 51 of the glycosyl hydrolase classification system. However, ...[more]