Dataset Information

In silico multi-epitope Bunyumwera virus vaccine to target virus nucleocapsid N protein.

ABSTRACT:

Background

Bunyumwera virus can cause 82% mortality in humans currently with no vaccine or drugs for treatment. We described an in silico multi-epitope vaccine targeting Bunyumwera virus nucleocapsid N-protein and predicted B and T cell epitopes for immunogenicity, allergenicity, toxicity, and conservancy. For creating the most potent immunological response possible, docking epitopes with HLA alleles are chosen to screen them. The 3D vaccination was docked with the Toll-like receptor-8 using molecular dynamic simulations. To ensure production efficiency, the vaccine sequence was further cloned in silico in a plasmid pIB2 vector. For efficacy and safety, results must be supported in vitro and in vivo.

Results

The vaccine was cloned to enable expression and translation in a plasmid vector pIB2. It was expected to be antigenic, non-allergenic, and have a high binding affinity with TLR-8 in silico cloning. This multi-epitope vaccination may stimulate both innate and adaptive immunity.

Conclusion

The vaccine developed in this work was based on the nucleocapsid N-protein of the Bunyumwera virus and was created using a reverse vaccinology method. Further experimental validation is required to assess the vaccine's therapeutic effectiveness and immunogenicity.

SUBMITTER: Nelluri KDD

PROVIDER: S-EPMC9208256 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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In silico multi-epitope Bunyumwera virus vaccine to target virus nucleocapsid N protein.

Nelluri Kanaka Durga Devi KDD Ammulu Manne Anupama MA Durga M Lakshmi ML Sravani Melika M Kumar Vemuri Praveen VP Poda Sudhakar S

Journal, genetic engineering & biotechnology 20220620 1

<h4>Background</h4>Bunyumwera virus can cause 82% mortality in humans currently with no vaccine or drugs for treatment. We described an in silico multi-epitope vaccine targeting Bunyumwera virus nucleocapsid N-protein and predicted B and T cell epitopes for immunogenicity, allergenicity, toxicity, and conservancy. For creating the most potent immunological response possible, docking epitopes with HLA alleles are chosen to screen them. The 3D vaccination was docked with the Toll-like receptor-8 u ...[more]

PMID: 35723807

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Dataset Information

In silico multi-epitope Bunyumwera virus vaccine to target virus nucleocapsid N protein.

Background

Results

Conclusion

Publications

In silico multi-epitope Bunyumwera virus vaccine to target virus nucleocapsid N protein.

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