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Dual-layered hydrogels allow complete genome recovery with nucleic acid cytometry.

ABSTRACT: Targeting specific cells for sequencing is important for applications in cancer, microbiology, and infectious disease. Nucleic acid cytometry (NAC) is a powerful approach for accomplishing this because it allows specific cells to be isolated based on sequence biomarkers that are otherwise impossible to detect. However, existing methods require specialized microfluidic devices, limiting adoption. Here, a modified workflow is described that uses particle-templated emulsification (PTE) and flow cytometry to conduct the essential steps of cell detection and sorting normally accomplished by microfluidics. Our microfluidic-free workflow allows facile isolation and sequencing of cells, viruses, and nucleic acids and thus provides a powerful enrichment approach for targeted sequencing applications.

SUBMITTER: Hatori MN

PROVIDER: S-EPMC9208836 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Dual-layered hydrogels allow complete genome recovery with nucleic acid cytometry.

Hatori Makiko N MN Modavi Cyrus C Xu Peng P Weisgerber Daniel D Abate Adam R AR

Biotechnology journal 20220210 4

Targeting specific cells for sequencing is important for applications in cancer, microbiology, and infectious disease. Nucleic acid cytometry (NAC) is a powerful approach for accomplishing this because it allows specific cells to be isolated based on sequence biomarkers that are otherwise impossible to detect. However, existing methods require specialized microfluidic devices, limiting adoption. Here, a modified workflow is described that uses particle-templated emulsification (PTE) and flow cyt ...[more]

PMID: 35088927

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